个人简介

翁小成，教授，博士生导师，国家基金委优青获得者，湖北省楚天学者计划楚天学子。2020年湖北省科学技术奖励自然科学一等奖(核酸的分子识别和调控，第2完成人)。2018年获批国家自然科学基金优秀青年基金资助。2015年湖北省楚天学者计划楚天学子。2010年湖北省科学技术奖励自然科学奖一等奖（以核酸为靶目标的小分子的设计、合成及其生物活性研究，第2完成人）。获武汉大学第五届青年教师教学竞赛一等奖(2010年)。获英国皇家化学会RSC “Young Chemist Award”参会项目资助(2009年)。已主持国家自然科学基金面上项目4项，青年基金1项，承担国家基金委重大研究计划“基因信息传递过程中非编码RNA的调控作用机制”集成项目课题1项。发表SCI论文80多篇，其中以共同第一/通讯作者在Nat. Chem. Biol.,Cancer Cell,Nat. Commun.,J. Am. Chem. Soc.,Angew. Chem. Int. Ed.等刊物上发表论文30多篇。

教育经历

1998.09-2002.07     武汉大学化学与分子科学学院，化学基地班，学士

2002.07-2007.11     武汉大学化学与分子科学学院，有机化学，博士，导师：周翔教授

工作经历

2007.11-2010.11     武汉大学化学与分子科学学院，讲师

2010.11-2018.09     武汉大学化学与分子科学学院，副教授

2013.11-2016.11     美国芝加哥大学 访问学者，博士后，导师：何川 教授

2018.09-至今         武汉大学化学与分子科学学院，教授

研究方向

长期从事核酸化学生物学的研究。基于化学探针等化学技术，融合分子生物学、基因组学、细胞生物学等手段，开发核酸表观遗传修饰、核酸结构以及核酸/蛋白质相互作用的研究技术，并开展相应的功能研究，以及开拓在临床疾病诊断方面的应用。

代表成果

1. Ruitu Lyu, Tong Wu, Allen C. Zhu, Diana C. West-Szymanski,Xiaocheng Weng, Mengjie Chen, Chuan He*. KAS-seq: genome-wide sequencing of single-stranded DNA by N3-kethoxal-assisted labeling.Nature Protocols,2021,accepted.

2. Yafen Wang, Shaoqing Han, Ruoxi Ran, Anling Li, Huanyu Liu, Mingjun Liu, Yongwei Duan, Xiong Zhang, Zhigang Zhao, Shihui Song,Xiaocheng Weng*, Songmei Liu*, Xiang Zhou*, A longitudinal sampling study of transcriptomic and epigenetic profiles in patients with thrombocytopenia syndrome.Nature Communications2021,12, 5629.

3. Wang, Y. F.; Zhang, X.; Han, S. Q.; Yang, W.; Chen, Z. G.; Wu, F.; Liu, J. Z.;Weng, X. C.*; Zhou, X.*, Base-Resolution Analysis of Deoxyuridine at the Genome Scale Based on the Artificial Incorporation Modified Nucleobase.Acs Central Science2021,7(6), 973-979. (封面文章)

4. Wang, Q.; Tan, K. Y.; Wang, H.; Shang, J. H.; Wan, Y. Q.; Liu, X. Q.;Weng, X. C.; Wang, F. A.*, Orthogonal Demethylase-Activated Deoxyribozyme for Intracellular Imaging and Gene Regulation.Journal of the American Chemical Society2021,143 (18), 6895-6904.

5. Wang, B. Y.; Zhang, X.; Wang, Y. F.; Chen, K.; Wang, F.;Weng, X. C.*; Zhou, X.*, One-pot fluorescent assay for sensitive detection of APOBEC3A activity.RSC Chemical Biology2021, 2 (4), 1201-1205.

6. Wang, B. Y.; Chen, Y.; Zhang, X.; Jiang, Z. R.; Wang, Y. F.; Chen, K.; Wang, F.;Weng, X. C.*; Zhou, X.*, A far-red emissive two-photon fluorescent probe for quantification of uracil in genomic DNA.Chemical Communications2021,57 (22), 2784-2787.

7. Li, W.; Yang, H. M.; Wang, Y. F.;Weng, X. C.*; Wang, F.*, Highly sensitive detection of 6mA at single-base resolution based on A-C mismatch.Analyst2021,146 (14), 4450-4453.

8. Huang, J. G.; Zhao, R. Q.; Mo, J.; Wang, F.;Weng, X. C.*; Zhou, X.*, N-3-Kethoxal-Based Bioorthogonal Intracellular RNA Labeling.Chembiochem2021,22 (9), 1559-1562.

9. Zhang, Y. J.; Zhang, Q. Y.;Weng, X. C.; Du, Y. H.*; Zhou, X., NEase-based amplification for detection of miRNA, multiple miRNAs and circRNA.Analytica Chimica Acta2021,1145, 52-58.

10. Huang, J. G.; Zhao, R. Q.; Qin, S. S.; Yang, S. X.;Li, W.; Mo, J.; Wang, F.; Du, Y. H.;Weng, X. C.*; Zhou, X., 4-Thiouridine-Enhanced Peroxidase-Generated Biotinylation of RNA.Chembiochem2021,22, 212-216.

11. Mo, J.; Chen, Z. G.; Qin, S. S.; Li, S.; Liu, C. G.; Zhang, L.; Ran, R. X.; Kong, Y.; Wang, F.; Liu, S. M.; Zhou, Y.; Zhang, X. L.;Weng, X. C.*; Zhou, X.*, TRADES: Targeted RNA Demethylation by SunTag System.Advanced Science2020,7 (19),202001402.

12. Chen, Yuqi; Wu, Fan; Chen, Zonggui; He, Zhiyong; Wei, Qi; Zeng, Weiwu; Chen, Kun; Xiao, Feng; Yuan, Yushu;Weng, Xiaocheng; Zhou, Yu; Zhou, Xiang*, Acrylonitrile-Mediated Nascent RNA Sequencing for Transcriptome-Wide Profiling of Cellular RNA Dynamics.Advanced Science2020,7 (8), 1900997.

13.Weng, X.; Gong, J.; Chen, Y.; Wu, T.; Wang, F.; Yang, S.; Yuan, Y.; Luo, G.; Chen, K.; Hu, L.; Ma, H.; Wang, P.; Zhang, Q. C.*; Zhou, X.*; He, C.*, Keth-seq for transcriptome-wide RNA structure mapping.Nature Chemical Biology2020,16(5), 489-492.

14. Yang, S.; Wu, F.; Peng, S.; Wang, F.; Chen, Y.; Yuan, Y.;Weng, X.*; Zhou, X., A m6A sensing method by its impact on the stability of RNA double helix.Chemistry undefinedamp; Biodiversity2020,https://doi.org/10.1002/cbdv.202000050.

15. Liu, Y. Q.; Zhang, X.; Liu, M.; Xu, F.; Zhang, Q. Y.; Zhang, Y. J.;Weng, X. C.; Liu, S. M.; Du, Y. H.*; Zhou, X.*, Direct detection of circRNA in real samples using reverse transcription-rolling circle amplification.Analytica Chimica Acta2020,1101, 169-175.

16. Zou, G. R.; Liu, C. X.; Fang, Z. T.; Jia, S. K.; Yang, W.; Zhang, X.; Chen, M. K.; Tian, T.;Weng, X. C.; Zhou, X.*, Photocaged probes for spatiotemporal imaging.Sensors and Actuators B-Chemical2019,288, 113-119.

17. Li, W.; Zeng, W. W.; Chen, Y.; Wang, F.; Wu, F.;Weng, X. C.*; Zhou, X.*, Biotinylation and isolation of an RNA G-quadruplex based on its peroxidase-mimicking activity.Analyst2019,144(15), 4472-4476.

18. Li, W.; Wang, F.; Chen, Y.;Weng, X. C.*; Zhou, X., A sensitive and radiolabeling-free method for pseudouridine detection.Analytical Biochemistry2019,581.

19. Chen, Y.; Yang, S.; Peng, S.; Li, W.; Wu, F.; Yao, Q.; Wang, F.;Weng, X.*; Zhou, X.*, N1-methyladenosine detection with CRISPR-Cas13a/C2c2.Chemical Science2019,10, 2975-2979

20. Zhong, X.; Yu, J. Y.; Frazier, K.;Weng, X. C.; Li, Y.; Cham, C. M.; Dolan, K.; Zhu, X. R.; Hubert, N.; Tao, Y.; Lin, F. F.; Martinez-Guryn, K.; Huang, Y.; Wang, T.; Liu, J. Z.; He, C.*; Chang, E. B.*; Leone, V.*, Circadian Clock Regulation of Hepatic Lipid Metabolism by Modulation of m(6)A mRNA Methylation.Cell Reports2018,25(7), 1816-+.